Scientists from Institute of fundamental biology and biotechnology of SFU and Institute of biophysics of KNTs Siberian Branch of the Russian Academy of Science presented qualitatively new development which is cheaper and more precisely than other analogs.
"Among modern biotests not many can present speed and easy performance. For example, analysis duration by biotests on the basis of the lyophilized bacteria — Lumistox (Hach, United kingdom), Microtox (Modern water, United States) is about an hour. And the biotest based on use of eggs of Crustacea of Thamnocephalus platyurus yields result in 30 minutes. Bioluminescent enzymatic biotests — champions on the speed and quality of assessment. Though today they are applied generally to testing of water and water solutions, we believe that these test systems are effective also for assessment of safety of multicomponent samples — soils, biological liquids, food" — reported the associate professor of biophysics, the research associate of laboratory of the SFU bioluminescent technologies Elena Esimbekova.
It is possible to minimize risk for health of consumers thanks to simple express methods of biotesting. They will help not only to reveal the number of the polluting connections in environments, but also adequately to estimate toxic influence which these connections can render on live systems.
"The research objective was to develop the universal scheme allowing to design the new biotests suitable for assessment of safety of natural and artificial circles of complex structure. As "developer" of potential danger we used the bifermentny system of the shining bacteria OVER (T) •Н: FMN-oxidoreductase of a lyutsiferaz (R L). In other words, if by means of the test it is possible to reveal potentially dangerous substance — it influences enzymatic reactions and changes intensity of the let-out light. In general advantage of enzymatic methods of the toxicological analysis that it is possible to increase sensitivity of an enzymatic system to effect of potentially dangerous substances, varying conditions of carrying out the analysis (for example, changing concentration of enzymes and/or substrates in reactionary mix)" — the manager of department of biophysics of SFU Valentina Kratasyuk explained.
During the experiments scientists managed to show that the R L bifermentny system shows high sensitivity to a number of metals: to lead, zinc, copper, mercury, aluminum and chrome. The same way investigated influence of 4 pesticides, the press service of SFU reports.
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дата публикации: 05 Июня 2019 г.